Difference between revisions of "DnaC"

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(References)
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* '''Description:''' replicative DNA helicase <br/><br/>
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* '''Description:''' replicative DNA helicase, part of the [[replisome]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[DnaB]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11250911 PubMed],  [[DnaI]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed]
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* '''Interactions:''' [[DnaB]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11250911 PubMed],  [[DnaI]]-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed], part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}}
  
 
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
 
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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=References=
 
=References=
  
<pubmed>10844689,12718886,11250911,,16479537, 10844689 19744498 19968790 </pubmed>
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<pubmed>10844689,12718886,11250911, 20122408,16479537, 10844689 19744498 19968790 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:22, 4 February 2010

  • Description: replicative DNA helicase, part of the replisome

Gene name dnaC
Synonyms
Essential yes PubMed
Product replicative DNA helicase
Function DNA replication
MW, pI 50 kDa, 4.548
Gene length, protein length 1362 bp, 454 aa
Immediate neighbours yycE, yycD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DnaC context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU40440

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: DnaB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: Cytoplasm (Homogeneous) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

Wiep Klaas Smits, Alexi I Goranov, Alan D Grossman
Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo.
Mol Microbiol: 2010, 75(2);452-61
[PubMed:19968790] [WorldCat.org] [DOI] (I p)

Kuang-Lei Tsai, Yu-Hua Lo, Yuh-Ju Sun, Chwan-Deng Hsiao
Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.
J Mol Biol: 2009, 393(5);1056-69
[PubMed:19744498] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Marion Velten, Stephen McGovern, Stéphanie Marsin, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
A two-protein strategy for the functional loading of a cellular replicative DNA helicase.
Mol Cell: 2003, 11(4);1009-20
[PubMed:12718886] [WorldCat.org] [DOI] (P p)

M Bárcena, T Ruiz, L E Donate, S E Brown, N E Dixon, M Radermacher, J M Carazo
The DnaB.DnaC complex: a structure based on dimers assembled around an occluded channel.
EMBO J: 2001, 20(6);1462-8
[PubMed:11250911] [WorldCat.org] [DOI] (P p)

Y Imai, N Ogasawara, D Ishigo-Oka, R Kadoya, T Daito, S Moriya
Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids.
Mol Microbiol: 2000, 36(5);1037-48
[PubMed:10844689] [WorldCat.org] [DOI] (P p)