Difference between revisions of "RpoE"

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(Expression and regulation)
(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
 
+
* '''Operon:'''  
* '''Operon:''' ''[[fadF]]-[[acdA]]-[[rpoE]]''
+
** ''[[fadF]]-[[acdA]]-[[rpoE]]'' {{PubMed|17189250}}
 +
** ''[[rpoE]]'' {{PubMed|10336502}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
 +
** ''[[fadF]]'': [[SigA]] {{PubMed|17189250}}
 +
** ''[[rpoE]]'': [[SigA]] {{PubMed|10336502}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** ''[[fadF]]'': repressed in the absence of long-chain fatty acids ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
+
** ''[[fadF]]'': repressed in the absence of long-chain fatty acids ([[FadR]]) {{PubMed|17189250}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[FadR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
+
** [[FadR]]: transcription repression {{PubMed|17189250}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 19:23, 3 February 2010

  • Description: RNA polymerase delta subunit

Gene name rpoE
Synonyms
Essential no
Product RNA polymerase delta subunit
Function transcription
MW, pI 20 kDa, 3.654
Gene length, protein length 519 bp, 173 aa
Immediate neighbours pyrG, acdA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RpoE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37160

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: rpoE family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • fadF: repressed in the absence of long-chain fatty acids (FadR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

Your additional remarks

References

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

F J López de Saro, N Yoshikawa, J D Helmann
Expression, abundance, and RNA polymerase binding properties of the delta factor of Bacillus subtilis.
J Biol Chem: 1999, 274(22);15953-8
[PubMed:10336502] [WorldCat.org] [DOI] (P p)

F J López de Saro, A Y Woody, J D Helmann
Structural analysis of the Bacillus subtilis delta factor: a protein polyanion which displaces RNA from RNA polymerase.
J Mol Biol: 1995, 252(2);189-202
[PubMed:7545758] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
Pathway of promoter melting by Bacillus subtilis RNA polymerase at a stable RNA promoter: effects of temperature, delta protein, and sigma factor mutations.
Biochemistry: 1995, 34(26);8465-73
[PubMed:7599136] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
The delta subunit of Bacillus subtilis RNA polymerase. An allosteric effector of the initiation and core-recycling phases of transcription.
J Mol Biol: 1994, 239(1);1-14
[PubMed:7515111] [WorldCat.org] [DOI] (P p)

M Lampe, C Binnie, R Schmidt, R Losick
Cloned gene encoding the delta subunit of Bacillus subtilis RNA polymerase.
Gene: 1988, 67(1);13-9
[PubMed:2843435] [WorldCat.org] [DOI] (P p)