Difference between revisions of "CotH"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (inner) | |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (inner) | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || protection of CotU and CotC in the mother cell | + | |style="background:#ABCDEF;" align="center"|'''Function''' || protection of [[CotU]] and [[CotC]] in the mother cell |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 5.953 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 5.953 | ||
| Line 88: | Line 88: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[cotH]]'' {{PubMed|8755863}} |
| − | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190}} | + | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15699190,8755863}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|15699190,8755863,15470121}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[GerE]]: transcription repression {{PubMed|15470121}} | ||
| + | |||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 118: | Line 121: | ||
=References= | =References= | ||
| − | <pubmed>10198031,, </pubmed> | + | <pubmed>10198031,15699190,8755863,15470121, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 21:52, 29 January 2010
- Description: spore coat protein (inner)
| Gene name | cotH |
| Synonyms | ywrH |
| Essential | no |
| Product | spore coat protein (inner) |
| Function | protection of CotU and CotC in the mother cell |
| MW, pI | 42 kDa, 5.953 |
| Gene length, protein length | 1086 bp, 362 aa |
| Immediate neighbours | cotB, cotG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU36060
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cotH family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: spore wall (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q45535
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Loredana Baccigalupi, Gaetano Castaldo, Giuseppina Cangiano, Rachele Isticato, Rosangela Marasco, Maurilio De Felice, Ezio Ricca
GerE-independent expression of cotH leads to CotC accumulation in the mother cell compartment during Bacillus subtilis sporulation.
Microbiology (Reading): 2004, 150(Pt 10);3441-9
[PubMed:15470121]
[WorldCat.org]
[DOI]
(P p)
R Zilhão, G Naclerio, A O Henriques, L Baccigalupi, C P Moran, E Ricca
Assembly requirements and role of CotH during spore coat formation in Bacillus subtilis.
J Bacteriol: 1999, 181(8);2631-3
[PubMed:10198031]
[WorldCat.org]
[DOI]
(P p)
G Naclerio, L Baccigalupi, R Zilhao, M De Felice, E Ricca
Bacillus subtilis spore coat assembly requires cotH gene expression.
J Bacteriol: 1996, 178(15);4375-80
[PubMed:8755863]
[WorldCat.org]
[DOI]
(P p)
