Difference between revisions of "ThdF"

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(Additional information)
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=== Additional information===
 
=== Additional information===
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Information on the corresponding protein from ''E. coli'': [http://BioCyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10997-MONOMER EcoCyc]
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MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with [[GidA]]. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.
  
 
=Expression and regulation=
 
=Expression and regulation=

Revision as of 18:06, 7 January 2010

  • Description: GTP-binding protein, putative tRNA modification GTPase

Gene name thdF
Synonyms
Essential no
Product putative tRNA modification GTPase
Function tRNA modification
MW, pI 50 kDa, 4.643
Gene length, protein length 1377 bp, 459 aa
Immediate neighbours gidA, jag
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThdF jag spoIIIJ rnpA rpmH context.png
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU41020

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: MnmE subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Information on the corresponding protein from E. coli: EcoCyc MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References