Hag

• Description: flagellin protein
  
  

Gene name	hag
Synonyms
Essential	no
Product	flagellin protein
Function	movement and chemotaxis
MW, pI	32 kDa, 4.782
Gene length, protein length	912 bp, 304 aa
Immediate neighbours	yvyC, csrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial flagellin family (according to Swiss-Prot)

• Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: secreted (according to Swiss-Prot), extracellular (no signal peptide) PubMed, membrane PubMed

Database entries

• Structure:

• UniProt: P02968

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hag

• Sigma factor: SigD

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • CsrA: translation inhibition PubMed

• Additional information:

Biological materials

• Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab

• Expression vector:

• lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: