NrgA

• Description: ammonium transporter, required at low ammonium concentration
  
  

Gene name	nrgA
Synonyms	amtB
Essential	no
Product	ammonium transporter
Function	ammonium uptake
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	42 kDa, 5.727
Gene length, protein length	1212 bp, 404 aa
Immediate neighbours	ywoB, nrgB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36510

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrgA-NrgB PubMed

• Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

• Structure: 2NUU (the complex with NrgB, from E. coli) PubMed

• UniProt: Q07429

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB

• Sigma factor: SigA

• Regulation: induced by ammonium limitation
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism: transcription activation by TnrA
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP739 (cat), GP255 (nrgAB, cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP168 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage