Difference between revisions of "YbgE"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
(Biological materials)
Line 109: Line 109:
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP248 (in [[pAC5]]), available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 19:14, 26 October 2009

  • Description: branched-chain amino acid aminotransferase

Gene name ybgE
Synonyms
Essential no
Product branched-chain amino acid aminotransferase
Function biosynthesis of branched-chain amino acids
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val, Coenzyme A
MW, pI 39 kDa, 4.952
Gene length, protein length 1068 bp, 356 aa
Immediate neighbours ybgB, ybgF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbgE context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU02390

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)
  • Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s): YwaA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP248 (in pAC5), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References