Difference between revisions of "CotE"
| Line 88: | Line 88: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[cotE]]'' | + | * '''Operon:''' ''[[cotE]]'' {{PubMed|8760914}} |
| − | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190}} | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,1691789}}, [[SigK]] {{PubMed|1691789}} |
| − | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' |
| + | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
| + | ** expressed during sporulation in the mother cell ([[SigE]], [[SigK]]) {{PubMed|1691789}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| Line 122: | Line 124: | ||
=References= | =References= | ||
| − | <pubmed>8748030,17720779,10559171 7592342,3139490,8299942,1691789,12644503,17172339,19304857,11737650 ,12107147, </pubmed> | + | <pubmed>8748030,17720779,10559171 8760914 15699190 7592342,3139490,8299942,1691789,12644503,17172339,19304857,11737650 ,12107147, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:59, 16 October 2009
- Description: spore coat protein (outer)
| Gene name | cotE |
| Synonyms | |
| Essential | no |
| Product | spore coat protein (outer) |
| Function | required for assembly of CotC-CotU complex |
| MW, pI | 20 kDa, 4.139 |
| Gene length, protein length | 543 bp, 181 aa |
| Immediate neighbours | ymcA, mutS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU17030
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cotE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: homotypic interaction PubMed
- Localization: spore wall (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P14016
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
Adriano Henriques, Lisbon, Portugal homepage
Your additional remarks
References
Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857]
[WorldCat.org]
[DOI]
(I p)
Caitlin C Ferguson, Amy H Camp, Richard Losick
gerT, a newly discovered germination gene under the control of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(21);7681-9
[PubMed:17720779]
[WorldCat.org]
[DOI]
(P p)
Teresa Costa, Mónica Serrano, Leif Steil, Uwe Völker, Charles P Moran, Adriano O Henriques
The timing of cotE expression affects Bacillus subtilis spore coat morphology but not lysozyme resistance.
J Bacteriol: 2007, 189(6);2401-10
[PubMed:17172339]
[WorldCat.org]
[DOI]
(P p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Katerina Ragkousi, Patrick Eichenberger, Christiaan van Ooij, Peter Setlow
Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate.
J Bacteriol: 2003, 185(7);2315-29
[PubMed:12644503]
[WorldCat.org]
[DOI]
(P p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
S Little, A Driks
Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.
Mol Microbiol: 2001, 42(4);1107-20
[PubMed:11737650]
[WorldCat.org]
[DOI]
(P p)
T Bauer, S Little, A G Stöver, A Driks
Functional regions of the Bacillus subtilis spore coat morphogenetic protein CotE.
J Bacteriol: 1999, 181(22);7043-51
[PubMed:10559171]
[WorldCat.org]
[DOI]
(P p)
F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914]
[WorldCat.org]
[DOI]
(P p)
K Pogliano, E Harry, R Losick
Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy.
Mol Microbiol: 1995, 18(3);459-70
[PubMed:8748030]
[WorldCat.org]
[DOI]
(P p)
C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342]
[WorldCat.org]
[DOI]
(P p)
A Driks, S Roels, B Beall, C P Moran, R Losick
Subcellular localization of proteins involved in the assembly of the spore coat of Bacillus subtilis.
Genes Dev: 1994, 8(2);234-44
[PubMed:8299942]
[WorldCat.org]
[DOI]
(P p)
L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789]
[WorldCat.org]
[DOI]
(P p)
L B Zheng, W P Donovan, P C Fitz-James, R Losick
Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore.
Genes Dev: 1988, 2(8);1047-54
[PubMed:3139490]
[WorldCat.org]
[DOI]
(P p)
