Difference between revisions of "SpoIVFB"

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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' processing of pro-sigma-K to active [[SigK]]  
+
* '''Catalyzed reaction/ biological activity:''' processing of pro-sigma-K to active [[SigK]] {{PubMed|19805276}}
  
 
* '''Protein family:''' peptidase M50B family (according to Swiss-Prot)
 
* '''Protein family:''' peptidase M50B family (according to Swiss-Prot)
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* '''Domains:'''  
 
* '''Domains:'''  
 +
** C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP {{PubMed|19805276}}
  
 
* '''Modification:'''
 
* '''Modification:'''
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* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
* '''Effectors of protein activity:'''
+
* '''Effectors of protein activity:''' ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level  {{PubMed|19805276}}
  
* '''Interactions:''' [[SpoIVFB]]-pro[[SigK]]
+
* '''Interactions:''' [[SpoIVFB]]-pro[[SigK]] {{PubMed|19805276}}
  
 
* '''Localization:''' cell membrane
 
* '''Localization:''' cell membrane
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=References=
 
=References=
  
<pubmed>11959848,9501233,12940997,1577688,12060714,9078383,1942049,10611287, 16818230 </pubmed>
+
<pubmed>11959848,9501233,12940997,1577688,12060714,9078383,1942049,10611287, 16818230 19805276 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:54, 8 October 2009

  • Description: intramembrane metalloprotease, processing of pro-sigma-K to active SigK

Gene name spoIVFB
Synonyms
Essential no
Product intramembrane metalloprotease
Function processing of pro-sigma-K to active SigK
MW, pI 33 kDa, 8.483
Gene length, protein length 864 bp, 288 aa
Immediate neighbours rplU, spoIVFA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIVFB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU27970

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: processing of pro-sigma-K to active SigK PubMed
  • Protein family: peptidase M50B family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level PubMed
  • Localization: cell membrane

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ruanbao Zhou, Christina Cusumano, Dexin Sui, R Michael Garavito, Lee Kroos
Intramembrane proteolytic cleavage of a membrane-tethered transcription factor by a metalloprotease depends on ATP.
Proc Natl Acad Sci U S A: 2009, 106(38);16174-9
[PubMed:19805276] [WorldCat.org] [DOI] (I p)

Nathalie Campo, David Z Rudner
A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.
Mol Cell: 2006, 23(1);25-35
[PubMed:16818230] [WorldCat.org] [DOI] (P p)

Tran C Dong, Simon M Cutting
SpoIVB-mediated cleavage of SpoIVFA could provide the intercellular signal to activate processing of Pro-sigmaK in Bacillus subtilis.
Mol Microbiol: 2003, 49(5);1425-34
[PubMed:12940997] [WorldCat.org] [DOI] (P p)

David Z Rudner, Qi Pan, Richard M Losick
Evidence that subcellular localization of a bacterial membrane protein is achieved by diffusion and capture.
Proc Natl Acad Sci U S A: 2002, 99(13);8701-6
[PubMed:12060714] [WorldCat.org] [DOI] (P p)

David Z Rudner, Richard Losick
A sporulation membrane protein tethers the pro-sigmaK processing enzyme to its inhibitor and dictates its subcellular localization.
Genes Dev: 2002, 16(8);1007-18
[PubMed:11959848] [WorldCat.org] [DOI] (P p)

D Z Rudner, P Fawcett, R Losick
A family of membrane-embedded metalloproteases involved in regulated proteolysis of membrane-associated transcription factors.
Proc Natl Acad Sci U S A: 1999, 96(26);14765-70
[PubMed:10611287] [WorldCat.org] [DOI] (P p)

O Resnekov, R Losick
Negative regulation of the proteolytic activation of a developmental transcription factor in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1998, 95(6);3162-7
[PubMed:9501233] [WorldCat.org] [DOI] (P p)

O Resnekov, S Alper, R Losick
Subcellular localization of proteins governing the proteolytic activation of a developmental transcription factor in Bacillus subtilis.
Genes Cells: 1996, 1(6);529-42
[PubMed:9078383] [WorldCat.org] [DOI] (P p)

E Ricca, S Cutting, R Losick
Characterization of bofA, a gene involved in intercompartmental regulation of pro-sigma K processing during sporulation in Bacillus subtilis.
J Bacteriol: 1992, 174(10);3177-84
[PubMed:1577688] [WorldCat.org] [DOI] (P p)

S Cutting, S Roels, R Losick
Sporulation operon spoIVF and the characterization of mutations that uncouple mother-cell from forespore gene expression in Bacillus subtilis.
J Mol Biol: 1991, 221(4);1237-56
[PubMed:1942049] [WorldCat.org] [DOI] (P p)