Difference between revisions of "TyrZ"

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(Biological materials)
(Expression and regulation)
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* '''Regulation:'''  
 
* '''Regulation:'''  
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** induced by tyrosine limitation ([[T-box]]) {{PubMed|19258532}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532}}
  
* '''Additional information:'''  
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* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 20:10, 1 October 2009

  • Description: tyrosyl-tRNA synthetase (minor)

Gene name tyrZ
Synonyms ipa-9r, tyrS1, tyrT
Essential no
Product tyrosyl-tRNA synthetase (minor)
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 46 kDa, 6.661
Gene length, protein length 1239 bp, 413 aa
Immediate neighbours ywaE, ywaD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TyrZ context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU38460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
  • Protein family: TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Additional information:

Biological materials

  • Mutant: QB53532 (aphA3), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)