ConE

• Description: conjugation protein of the integrative and conjugative element ICEBs1
  
  

Gene name	conE
Synonyms	yddE
Essential	no
Product	conjugation protein
Function	conjugative transfer of ICEBs1
MW, pI	96 kDa, 5.511
Gene length, protein length	2493 bp, 831 aa
Immediate neighbours	yddD, yddF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04940

Phenotypes of a mutant

Completely defective for conjugal transfer of ICEBs1

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: required for conjugative transfer of ICEBs1, putative ATPase PubMed

• Protein family: HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Located at the cell poles, in close association with the membrane

Database entries

• Structure:

• UniProt: P96642

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Melanie Berkmen, Suffolk University, Boston, MA

References

Melanie B Berkmen, Catherine A Lee, Emma-Kate Loveday, Alan D Grossman
Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
J Bacteriol: 2010, 192(1);38-45
[PubMed:19734305] [WorldCat.org] [DOI] (I p)