Difference between revisions of "PutC"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[ycgM]]-[[ycgN]]'' | + | * '''Operon:''' ''[[ycgM]]-[[ycgN]]'' (according to [http://dbtbs.hgc.jp/COG/prom/ycgMN.html DBTBS]) |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| − | * '''Regulation:''' repressed | + | * '''Regulation:''' |
| + | ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | ||
| + | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
| − | * '''Regulatory mechanism:''' [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | + | * '''Regulatory mechanism:''' |
| + | ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | ||
| + | ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 20:17, 5 August 2009
- Description: 1-pyrroline-5-carboxylate dehydrogenase
| Gene name | ycgN |
| Synonyms | |
| Essential | no |
| Product | 1-pyrroline-5-carboxylate dehydrogenase |
| Function | proline utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 56 kDa, 5.372 |
| Gene length, protein length | 1545 bp, 515 aa |
| Immediate neighbours | ycgM, ycgO |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU03210
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H (according to Swiss-Prot)
- Protein family: RocA subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P94391
- KEGG entry: [3]
- E.C. number: 1.5.1.12
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
