Pdp

• Description: pyrimidine nucleoside phosphorylase
  
  

Gene name	pdp
Synonyms
Essential	no
Product	pyrimidine nucleoside phosphorylase
Function	pyrimidine interconversion
Metabolic function and regulation of this protein in SubtiPathways: Nucleoside catabolism, Nucleotides (regulation)
MW, pI	46 kDa, 4.765
Gene length, protein length	1299 bp, 433 aa
Immediate neighbours	hutM, nupC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A pyrimidine nucleoside + phosphate = a pyrimidine base + alpha-D-ribose 1-phosphate (according to Swiss-Prot)

• Protein family: thymidine/pyrimidine-nucleoside phosphorylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1BRW (Geobacillus stearothermophilus)

• UniProt: P39142

• KEGG entry: [3]

• E.C. number: 2.4.2.2

Expression and regulation

• Operon:

• Sigma factor: SigK PubMed

• Regulation: repressed by glucose (CcpA)

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xianmin Zeng, Anne Galinier, Hans H Saxild
Catabolite repression of dra-nupC-pdp operon expression in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 11);2901-2908
[PubMed:11065368] [WorldCat.org] [DOI] (P p)

X Zeng, H H Saxild
Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis.
J Bacteriol: 1999, 181(6);1719-27
[PubMed:10074062] [WorldCat.org] [DOI] (P p)

H H Saxild, L N Andersen, K Hammer
Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein.
J Bacteriol: 1996, 178(2);424-34
[PubMed:8550462] [WorldCat.org] [DOI] (P p)