RecN

• Description: DNA repair and genetic recombination
  
  

Gene name	recN
Synonyms
Essential	no
Product	unknown
Function	DNA repair and genetic recombination
MW, pI	64 kDa, 4.802
Gene length, protein length	1728 bp, 576 aa
Immediate neighbours	spoIVB, ahrC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24240

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: recN family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore intermembrane space (according to Swiss-Prot), Nucleoid (Multiple) PubMed

Database entries

• Structure:

• UniProt: P17894

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Lyle A Simmons, Alexi I Goranov, Hajime Kobayashi, Bryan W Davies, Daniel S Yuan, Alan D Grossman, Graham C Walker
Comparison of responses to double-strand breaks between Escherichia coli and Bacillus subtilis reveals different requirements for SOS induction.
J Bacteriol: 2009, 191(4);1152-61
[PubMed:19060143] [WorldCat.org] [DOI] (I p)

Humberto Sanchez, Paula P Cardenas, Shige H Yoshimura, Kunio Takeyasu, Juan C Alonso
Dynamic structures of Bacillus subtilis RecN-DNA complexes.
Nucleic Acids Res: 2008, 36(1);110-20
[PubMed:17999999] [WorldCat.org] [DOI] (I p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Humberto Sanchez, Dawit Kidane, M Castillo Cozar, Peter L Graumann, Juan C Alonso
Recruitment of Bacillus subtilis RecN to DNA double-strand breaks in the absence of DNA end processing.
J Bacteriol: 2006, 188(2);353-60
[PubMed:16385024] [WorldCat.org] [DOI] (P p)

Humberto Sanchez, Juan C Alonso
Bacillus subtilis RecN binds and protects 3'-single-stranded DNA extensions in the presence of ATP.
Nucleic Acids Res: 2005, 33(7);2343-50
[PubMed:15849320] [WorldCat.org] [DOI] (I e)

B E Van Hoy, J A Hoch
Characterization of the spoIVB and recN loci of Bacillus subtilis.
J Bacteriol: 1990, 172(3);1306-11
[PubMed:2106508] [WorldCat.org] [DOI] (P p)