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| − | * '''Description:''' Carbon catabolite control protein A, involved in glucose regulation of many genes; represses catabolic genes and activates genes involved in excretion of excess carbon <br/><br/>
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''ccpA''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''graR, alsA, amyR''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || mediates carbon catabolite repression (CCR)
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/nucleosides_catabolism.html Nucleoside catabolism], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val],<br/>[http://subtiwiki.uni-goettingen.de/pathways/histidine.html His], [http://subtiwiki.uni-goettingen.de/pathways/CoA_synthesis.html Coenzyme A], [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36,8 kDa, 5.06
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1002 bp, 334 amino acids
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[aroA]]'', ''[[motP]]''
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14952]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:ccpA_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |}
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| − | __TOC__
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| − | <br/><br/><br/><br/><br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Locus tag:''' BSU29740
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| − | ===Phenotypes of a mutant ===
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| − | Loss of carbon catabolite repression.
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| − | Loss of PTS-dependent sugar transport due to excessive phosphorylation of [[PtsH |HPr]] by [[HprK]].
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| − | The mutant is unable to grow on a minimal medium with glucose and ammonium as the only sources of carbon and nitrogen, respectively.
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ccpA-motPS.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10376]
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| − | === Additional information===
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:''' transcriptional regulator of carbon catabolite repression (CCR)
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| − | * '''Protein family:''' LacI family
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| − | * '''Paralogous protein(s):'''
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| − | === Genes controlled by CcpA ===
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| − | * '''Activation by CcpA:''' ''[[pta]]'', ''[[ackA]]'', ''[[ilvB]]-[[ilvH]]-[[ilvC]]-[[leuA]]-[[leuB]]-[[leuC]]-[[leuD]]''
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| − | * '''Repression by CcpA:''' ''[[abbA]], [[amyE]]'', ''[[bglP]]-[[bglH]]'', ''[[bglS]]'', ''[[cccA]]'', ''[[citZ]]-[[icd]]-[[mdh]]'', ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'', ''[[licB]]-[[licC]]-[[licA]]-[[licH]]'', ''[[phoP]]-[[phoR]]'', ''[[xylA]]-[[xylB]]'', ''[[xynP]]-[[xynB]]''
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | ** HTH lacI-type Domain (1 – 58)
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| − | ** DNA binding Domain (6 – 25)
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):''' [[PtsH |HPr]]-Ser46-P, Crh-Ser-46-P
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| − | * '''Effectors of protein activity:'''glucose-6-phosphate, fructose-1,6-bisphosphate [http://www.ncbi.nlm.nih.gov/pubmed/17376479?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum Pubmed]
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| − | * '''Interactions:''' CcpA-[[PtsH |HPr]] [http://www.ncbi.nlm.nih.gov/pubmed/15369672?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed], CcpA-[[Crh]] [http://www.ncbi.nlm.nih.gov/pubmed/16316990?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed]
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| − | * '''Localization:'''
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| − | === Database entries ===
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| − | * '''Structure:''' CcpA-Crh-DNA-complex [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], complex with P-Ser-[[PtsH |HPr]] and sulphate ions [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39857 NCBI]
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| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P25144 P25144]
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29740]
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| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[ccpA]] [[motP]] [[motS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/16547058 PubMed]
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| − | * '''Sigma factor:'''
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| − | * '''Regulation:''' constitutively expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed]
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| − | * '''Additional information:''' there are about 3.000 molecules of CcpA per cell [http://www.ncbi.nlm.nih.gov/sites/entrez/8000527 PubMed]
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| − | =Biological materials =
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| − | * '''Mutant:''' QB5407 (spc), GP302 (erm), GP300 (an in frame deletion of ''[[ccpA]]''), available in [[Stülke]] lab
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| − | * '''Expression vector:''' pGP643 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''Antibody:''' available in [[Hillen]] and [[Stülke]] labs
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| − | =Labs working on this gene/protein=
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| − | [[Wolfgang Hillen]], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
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| − | [[Richard Brennan]], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
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| − | [[Milton H. Saier]], University of California at San Diego, USA [http://biology.ucsd.edu/faculty/saier.html Homepage]
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| − | [[Yasutaro Fujita]], University of Fukuyama, Japan
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| − | [[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| − | [[Oscar Kuipers]], University of Groningen, The Netherlands
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| − | [http://molgen.biol.rug.nl/molgen/index.php Homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | '''Reviews'''
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| − | <pubmed> 8598282 , 19202299,14665673,18628769 ,18359269, 18628769 </pubmed>
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| − | '''General and physiological studies'''
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| − | <pubmed>1904524 ,10941796 ,12123463,8000527, 18757537,16547058,14523131 </pubmed>
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| − | '''Global analyses (proteome, transcriptome)'''
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| − | <pubmed>12850135 ,11251851,10559165, 11160890,17183215 </pubmed>
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| − | '''Repression of target genes by CcpA'''
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| − | <pubmed>15150224 ,16166551 ,11929549 , 7913927 ,17827291 ,11985717 ,12100558,7592486 </pubmed>
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| − | '''Positive regulation of gene expression by CcpA'''
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| − | <pubmed>8226682 ,12193635 ,10559153 ,15916605, 9811655 ,10986270 </pubmed>
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| − | '''Control of CcpA activity'''
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| − | <pubmed>7623661 ,9973552 ,9334231 ,12051938, 9689125 </pubmed>
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| − | '''CcpA-DNA interaction'''
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| − | <pubmed>8596444 ,10666464 ,15885105,7665492 ,9254709 </pubmed>
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| − | '''Functional analysis of CcpA'''
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| − | <pubmed>10383986 ,10601226 ,11557150,9252590 ,9988473 </pubmed>
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| − | '''Structural analyses'''
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| − | <pubmed>15369672 ,16316990 ,17376479 </pubmed>
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