Difference between revisions of "BsrF"
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery | + | * '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the [[Sabine Brantl]] lab |
* '''Expression vectors:''' pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene) | * '''Expression vectors:''' pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene) | ||
| − | pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene) | + | pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene) available in the [[Sabine Brantl]] lab |
* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the [[Sabine Brantl]] lab | * '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the [[Sabine Brantl]] lab | ||
Revision as of 16:57, 14 July 2009
- Description: small RNA
| Gene name | bsrF |
| Synonyms | SR2 |
| Essential | no |
| Product | small RNA |
| Function | unknown |
| Gene length | bp |
| Immediate neighbours | yobO, csaA |
| Gene sequence (+200bp) | |
| Genetic context File:GenE context.gif | |
Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: no entry
- Structure:
Additional information
Expression and regulation
- Operon: bsrF PubMed
- Additional information:
Biological materials
- Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the Sabine Brantl lab
- Expression vectors: pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene)
pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene) available in the Sabine Brantl lab
- lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab
Labs working on this gene/RNA
- Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
- Kouji Nakamura, Tsukuba, University, Japan
Your additional remarks
References
Heike Preis, Rita A Eckart, Rajani K Gudipati, Nadja Heidrich, Sabine Brantl
CodY activates transcription of a small RNA in Bacillus subtilis.
J Bacteriol: 2009, 191(17);5446-57
[PubMed:19542274]
[WorldCat.org]
[DOI]
(I p)
Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis.
Gene: 2009, 428(1-2);2-8
[PubMed:18948176]
[WorldCat.org]
[DOI]
(P p)
