Difference between revisions of "ThiT"

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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32074 O32074]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32074 O32074]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU30990]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30990]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 09:46, 25 June 2009

  • Description: thiamine permease

Gene name thiT
Synonyms yuaJ
Essential no
Product thiamine permease
Function thiamine uptake
MW, pI 20 kDa, 10.237
Gene length, protein length 576 bp, 192 aa
Immediate neighbours rrnB-16S, yuaI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YuaJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU30990

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: proton-coupled thiamine transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)