Difference between revisions of "CheB"

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Line 82: Line 82:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q05522 Q05522]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q05522 Q05522]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16420]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16420]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.1.61 3.1.1.61]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.1.61 3.1.1.61]

Revision as of 01:29, 25 June 2009

  • Description: MCP-glutamate methylesterase / two-component response regulator-like

Gene name cheB
Synonyms cheL
Essential no
Product MCP-glutamate methylesterase / two-component response regulator-like
Function motility, chemotaxis
MW, pI 38 kDa, 8.119
Gene length, protein length 1071 bp, 357 aa
Immediate neighbours swrC, cheA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CheB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU16420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michael W Bunn, George W Ordal
Receptor conformational changes enhance methylesterase activity during chemotaxis by Bacillus subtilis.
Mol Microbiol: 2004, 51(3);721-8
[PubMed:14731274] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Michael A Zimmer, Hendrik Szurmant, Michael M Saulmon, Marissa A Collins, Jason S Bant, George W Ordal
The role of heterologous receptors in McpB-mediated signalling in Bacillus subtilis chemotaxis.
Mol Microbiol: 2002, 45(2);555-68
[PubMed:12123464] [WorldCat.org] [DOI] (P p)

J R Kirby, T B Niewold, S Maloy, G W Ordal
CheB is required for behavioural responses to negative stimuli during chemotaxis in Bacillus subtilis.
Mol Microbiol: 2000, 35(1);44-57
[PubMed:10632876] [WorldCat.org] [DOI] (P p)

D S Bischoff, G W Ordal
Sequence and characterization of Bacillus subtilis CheB, a homolog of Escherichia coli CheY, and its role in a different mechanism of chemotaxis.
J Biol Chem: 1991, 266(19);12301-5
[PubMed:1905718] [WorldCat.org] (P p)