PutB

• Description: proline oxidase (L-proline, NAD)
  
  

Gene name	ycgM
Synonyms
Essential	no
Product	proline oxidase (L-proline, NAD)
Function	proline utilization
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	34 kDa, 7.291
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	ycgL, ycgN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03200

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)

• Protein family: proline dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): FadM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P94390

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ycgM-ycgN

• Sigma factor:

• Regulation: repressed by CodY PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism: CodY: transcription repression PubMed
  • Spo0A: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)