Difference between revisions of "BioB"

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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P53557 P53557]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P53557 P53557]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU30200]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30200]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.8.1.6 2.8.1.6]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.8.1.6 2.8.1.6]

Revision as of 01:21, 25 June 2009

  • Description: biotin synthase

Gene name bioB
Synonyms
Essential no
Product biotin synthase)
Function biosynthesis of biotin
MW, pI 36 kDa, 5.554
Gene length, protein length 1005 bp, 335 aa
Immediate neighbours bioI, bioD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BioB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU30200

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot)
  • Protein family: Biotin synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)