Difference between revisions of "LeuB"
Line 125: | Line 125: | ||
<pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,,15547269,12618455,12107147, </pubmed> | <pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,,15547269,12618455,12107147, </pubmed> | ||
− | |||
− |
Revision as of 17:26, 15 June 2009
- Description: 3-isopropylmalate dehydrogenase
Gene name | leuB |
Synonyms | leuC |
Essential | no |
Product | 3-isopropylmalate dehydrogenase |
Function | biosynthesis of leucine |
MW, pI | 39 kDa, 4.744 |
Gene length, protein length | 1095 bp, 365 aa |
Immediate neighbours | leuC, leuA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU28270
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH (according to Swiss-Prot)
- Protein family: LeuB type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1XAD (Thermus thermophilus)
- Swiss prot entry: P05645
- KEGG entry: [3]
- E.C. number: 1.1.1.85
Additional information
Expression and regulation
- Regulation: repressed by casamino acids PubMed , expressed in the absence of branched-chain amino acids (BCAA), expression is stimulated in the presence of glucose PubMed, repressed by CodY PubMed
- Regulatory mechanism: CodY: transcription repression PubMed, glucose regulation: CcpA PubMed, repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References