Difference between revisions of "GapB"
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=== Extended information on the protein === | === Extended information on the protein === | ||
| − | * '''Kinetic information:''' | + | * '''Kinetic information:''' Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed] |
* '''Domains:''' | * '''Domains:''' | ||
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* '''Modification:''' | * '''Modification:''' | ||
| − | * '''Cofactor(s):''' | + | * '''Cofactor(s):''' NADP (preferentially) and NAD [http://www.ncbi.nlm.nih.gov/sites/entrez/10799476 PubMed] |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
Revision as of 12:29, 10 June 2009
- Description: glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme
| Gene name | gapB |
| Synonyms | ppc |
| Essential | no |
| Product | glyceraldehyde-3-phosphate dehydrogenase 2 |
| Function | anabolic enzyme in gluconeogenesis |
| MW, pI | 37,3 kDa, 6.47 |
| Gene length, protein length | 1020 bp, 340 amino acids |
| Immediate neighbours | ytcD, speD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU29020
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H (according to Swiss-Prot)
- Protein family: glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s): GapA
Extended information on the protein
- Kinetic information: Michaelis-Menten PubMed
- Domains:
- Nucleotid bindinge domain (12-13)
- 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)
- Modification:
- Cofactor(s): NADP (preferentially) and NAD PubMed
- Effectors of protein activity:
- Interactions:
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- Structure:
- Swiss prot entry: O34425
- KEGG entry: [3]
- E.C. number: 1.2.1.59
Additional information
Expression and regulation
- Regulatory mechanism: transcription repression
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References
