TreA

• Description: phospho-alpha-glucosidase
  
  

Gene name	treA
Synonyms	treC
Essential	no
Product	phospho-alpha-glucosidase
Function	trehalose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	65 kDa, 4.895
Gene length, protein length	1683 bp, 561 aa
Immediate neighbours	treP, treR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07810

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Alpha,alpha-trehalose 6-phosphate + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P39795

• KEGG entry: [3]

• E.C. number: 3.2.1.93 3

Expression and regulation

• Operon: treP-treA-treR

• Sigma factor: SigA

• Regulation: repressed by glucose (CcpA)

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant: GP1118 (spc), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

C Helfert, S Gotsche, M K Dahl
Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene.
Mol Microbiol: 1995, 16(1);111-120
[PubMed:7651129] [WorldCat.org] [DOI] (P p)

1. Helfert C, Gotsche S, Dahl MK. (1995) Cleavage of trehalose-phosphate in Bacillus subtilis is catalysed by a phospho-alpha-(1-1)-glucosidase encoded by the treA gene. Mol Microbiol. Apr;16(1): 111-120. PubMed