Difference between revisions of "SacA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of sucrose  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of sucrose  
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 54 kDa, 5.34   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 54 kDa, 5.34   

Revision as of 12:03, 11 June 2009

  • Description: phosphosucrase

Gene name sacA
Synonyms ipa-50d
Essential no
Product phosphosucrase
Function utilization of sucrose
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 54 kDa, 5.34
Gene length, protein length 1440 bp, 480 aa
Immediate neighbours ywdA, sacP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SacA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU38040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Fouet A, Klier A, Rapoport G. (1986) Nucleotide sequence of the sucrase gene of Bacillus subtilis. Gene 45(2): 221-5. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed