CccA

• Description: cytochrome c550
  
  

Gene name	cccA
Synonyms
Essential	no
Product	cytochrome c550
Function	respiration
MW, pI	12 kDa, 5.276
Gene length, protein length	360 bp, 120 aa
Immediate neighbours	yqfN, sigA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25190

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P24469

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repressed by glucose (4.8-fold) (CcpA) PubMed, carbon catabolite repression (CcpA) PubMed

• Regulatory mechanism: CcpA: transcription repression, CcpA: transcription repression, cre site overlaps -35 region of the promoter PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

V Monedero, G Boël, J Deutscher
Catabolite regulation of the cytochrome c550-encoding Bacillus subtilis cccA gene.
J Mol Microbiol Biotechnol: 2001, 3(3);433-8
[PubMed:11361075] [WorldCat.org] (P p)

P S David, M R Morrison, S L Wong, B C Hill
Expression, purification, and characterization of recombinant forms of membrane-bound cytochrome c-550nm from Bacillus subtilis.
Protein Expr Purif: 1999, 15(1);69-76
[PubMed:10024472] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Physico-chemical characterisation of membrane-bound and water-soluble forms of Bacillus subtilis cytochrome c-550.
Eur J Biochem: 1993, 212(2);499-509
[PubMed:8383048] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Bacillus subtilis holo-cytochrome c-550 can be synthesised in aerobic Escherichia coli.
FEBS Lett: 1990, 270(1-2);147-51
[PubMed:2171986] [WorldCat.org] [DOI] (P p)

C von Wachenfeldt, L Hederstedt
Bacillus subtilis 13-kilodalton cytochrome c-550 encoded by cccA consists of a membrane-anchor and a heme domain.
J Biol Chem: 1990, 265(23);13939-48
[PubMed:2166045] [WorldCat.org] (P p)

1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
2. Monedero, V., Boël, G., and Deutscher, J. (2001). Catabolite regulation of the cytochrome c550-encoding Bacillus subtilis cccA gene. J. Mol. Microbiol. Biotechnol. 3, 433-438. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed