Difference between revisions of "CysK"

From SubtiWiki
Jump to: navigation, search
(Phenotypes of a mutant)
Line 38: Line 38:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
constitutive expression of the ''[[cysH]]'' operon, auxotrophic for cysteine
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 10:15, 5 February 2009

  • Description: cysteine synthetase A

Gene name cysK
Synonyms
Essential
Product cysteine synthetase A
Function
MW, pI 32 kDa, 5.492
Gene length, protein length 924 bp, 308 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
CysK context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

constitutive expression of the cysH operon, auxotrophic for cysteine

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed


  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number: EC 4.2.99.8

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis.J. Biol. Chem. 283: 35551-35560. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed