LiaS

• Description: two-component sensor kinase, response to bacitracin
  
  

Gene name	liaS
Synonyms	yvqE
Essential	no
Product	two-component sensor kinase (NarL family)
Function	control of LiaR activity in response to bacitracin
MW, pI	40 kDa, 7.333
Gene length, protein length	1080 bp, 360 aa
Immediate neighbours	liaR, liaF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O32198

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: liaI-liaH-liaG-liaF-liaS-liaR

liaS-liaR

• Sigma factor: SigA

• Regulation: liaI: induced by bacitracin (LiaR)
  • repressed during logrithmic growth (AbrB) PubMed

liaS: constitutive

• Regulatory mechanism: LiaR: transcription activation
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Thorsten Mascher, Karlsruhe Institute of Technology, Germany link

References

Sina Jordan, Anja Junker, John D Helmann, Thorsten Mascher
Regulation of LiaRS-dependent gene expression in bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system.
J Bacteriol: 2006, 188(14);5153-66
[PubMed:16816187] [WorldCat.org] [DOI] (P p)

1. Jordan, S., A. Junker, J. D. Helmann, and T. Mascher. 2006. Regulation of LiaRS-dependent gene expression in Bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system. J. Bacteriol. 188: 5153-5166. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed