Difference between revisions of "Sandbox"
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− | * '''Description:''' | + | * '''Description:''' two-component response regulator, regulation of phosphate metabolism <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
− | |'' | + | |''phoP'' |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || two-component response regulator |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of phosphate metabolism <br/>(phoA, phoB, phoD, resABCDE, tagAB, tagDEF, tuaA-H) |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.068 |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 720 bp, 240 aa |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phoR]]'', ''[[mdh]]'' |
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS: | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14871]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
|- | |- | ||
|- | |- | ||
|- | |- | ||
− | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |colspan="2" | '''Genetic context''' <br/> [[Image:phoP_context.gif]] |
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
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__TOC__ | __TOC__ | ||
− | <br/><br/> | + | <br/><br/><br/><br/> |
=The gene= | =The gene= | ||
Line 37: | Line 37: | ||
=== Basic information === | === Basic information === | ||
− | * '''Locus tag:''' | + | * '''Locus tag:''' BSU29110 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
Line 43: | Line 43: | ||
=== Database entries === | === Database entries === | ||
− | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoPR.html] |
− | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10363] |
=== Additional information=== | === Additional information=== | ||
Line 54: | Line 54: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
− | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' |
− | * '''Protein family:''' | + | * '''Protein family:''' |
− | * '''Paralogous protein(s):''' [[ | + | * '''Paralogous protein(s):''' |
+ | |||
+ | === Genes controlled by PhoP === | ||
+ | |||
+ | * '''Activation by PhoP:''' ''[[phoP]]-[[phoR]]'' | ||
+ | |||
+ | * '''Repression by PhoP:''' | ||
=== Extended information on the protein === | === Extended information on the protein === | ||
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* '''Domains:''' | * '''Domains:''' | ||
− | * '''Modification:''' | + | * '''Modification:''' phosphorylation by [[PhoR]] under conditions of phosphate limitation (stimulates DNA-binding activity) |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
Line 72: | Line 78: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' | + | * '''Interactions:''' [[PhoP]]-[[PhoP]], [[PhoP]]-[[PhoR]] |
− | * '''Localization:''' | + | * '''Localization:''' cell membrane (according to Swiss-Prot) |
=== Database entries === | === Database entries === | ||
− | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MVO 1MVO] (receiver domain) |
− | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/ | + | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P13792 P13792] |
− | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29110] |
− | * '''E.C. number:''' | + | * '''E.C. number:''' |
=== Additional information=== | === Additional information=== | ||
Line 90: | Line 96: | ||
=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' ''[[ | + | * '''Operon:''' ''[[phoP]]-[[phoR]]'' |
− | * '''[[Sigma factor]]:''' [[SigA | + | * '''[[Sigma factor]]:''' [[SigA]] |
− | * '''Regulation:''' | + | * '''Regulation:''' repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed], induced by phosphate limitation ([[PhoP]]), carbon catabolite repression ([[CcpA]]) |
− | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, [[PhoP]]: transcription activation, [[CcpA]]: transcription repression |
* '''Additional information:''' | * '''Additional information:''' | ||
Line 121: | Line 127: | ||
=References= | =References= | ||
− | <pubmed> | + | <pubmed>12850135, </pubmed> |
− | # | + | # Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149 [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] |
− | |||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] |
Revision as of 13:29, 8 June 2009
- Description: two-component response regulator, regulation of phosphate metabolism
Gene name | phoP |
Synonyms | |
Essential | no |
Product | two-component response regulator |
Function | regulation of phosphate metabolism (phoA, phoB, phoD, resABCDE, tagAB, tagDEF, tuaA-H) |
MW, pI | 27 kDa, 5.068 |
Gene length, protein length | 720 bp, 240 aa |
Immediate neighbours | phoR, mdh |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU29110
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Genes controlled by PhoP
- Repression by PhoP:
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation by PhoR under conditions of phosphate limitation (stimulates DNA-binding activity)
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 1MVO (receiver domain)
- Swiss prot entry: P13792
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: repressed by glucose (4.3-fold) (CcpA) PubMed, induced by phosphate limitation (PhoP), carbon catabolite repression (CcpA)
- Regulatory mechanism: CcpA: transcription repression, PhoP: transcription activation, CcpA: transcription repression
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Marion Hulett, University of Illinois at Chicago, USA Homepage
Your additional remarks
References
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)