Difference between revisions of "Sandbox"

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−
* '''Description:''' transcription repressor of the fru operon <br/><br/>
+
* '''Description:''' UDP glucose 4-epimerase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''fruR''
+
|''galE''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor
+
|style="background:#ABCDEF;" align="center"| '''Product''' || UDP glucose 4-epimerase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of fructose utilization
+
|style="background:#ABCDEF;" align="center"|'''Function''' || galactose utilization  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.684  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 4.776  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 753 bp, 251 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1017 bp, 339 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yknZ]]'', ''[[fruK]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxkC]]'', ''[[yxkA]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13311&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15912&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:fruR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:galE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU14380
+
* '''Locus tag:''' BSU38860
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fruRKA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/galE.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12589]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11837]
  
 
=== Additional information===
 
=== Additional information===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' UDP-glucose = UDP-galactose (according to Swiss-Prot)
  
−
* '''Protein family:'''
+
* '''Protein family:''' sugar epimerase family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31713 O31713]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P55180 P55180]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14380]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38860]
  
−
* '''E.C. number:'''
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/5.1.3.2 5.1.3.2]
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''[[fruR]]-[[fruK]]-[[fruA]]''
+
* '''Operon:'''  
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
−
<pubmed>10627040, </pubmed>
+
<pubmed>9555917, </pubmed>
−
# Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''. ''Microbiology'' '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
+
# Krispin O, Allmansberger R. (1998) The Bacillus subtilis galE gene is essential in the presence of glucose and galactose. '' J Bacteriol.'' '''Apr;180(8):''' 2265-70. [http://www.ncbi.nlm.nih.gov/sites/entrez/9555917 PubMed]
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 13:17, 8 June 2009

  • Description: UDP glucose 4-epimerase

Gene name galE
Synonyms
Essential no
Product UDP glucose 4-epimerase
Function galactose utilization
MW, pI 36 kDa, 4.776
Gene length, protein length 1017 bp, 339 aa
Immediate neighbours yxkC, yxkA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GalE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU38860

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: UDP-glucose = UDP-galactose (according to Swiss-Prot)
  • Protein family: sugar epimerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

O Krispin, R Allmansberger
The Bacillus subtilis galE gene is essential in the presence of glucose and galactose.
J Bacteriol: 1998, 180(8);2265-70
[PubMed:9555917] [WorldCat.org] [DOI] (P p)

  1. Krispin O, Allmansberger R. (1998) The Bacillus subtilis galE gene is essential in the presence of glucose and galactose. J Bacteriol. Apr;180(8): 2265-70. PubMed
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