Difference between revisions of "Sandbox"

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* '''Description:''' cytochrome-c oxidase (subunit III) <br/><br/>
+
* '''Description:''' aspartokinase I (alpha and beta subunits) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ctaE''
+
|''dapG''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''lssD ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cytochrome-c oxidase (subunit III)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || aspartokinase I (alpha and beta subunits)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || respiration
+
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 7.411  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 5.709  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 621 bp, 207 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1212 bp, 404 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ctaD]]'', ''[[ctaF]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[asd]]'', ''[[dapA]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13364&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13549&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ctaE_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:dapG_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU14910
+
* '''Locus tag:''' BSU16760
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctaBCDEFG.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoVFAB-asd-dapG-dapA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10217]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10784]
  
 
=== Additional information===
 
=== Additional information===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' 4 ferrocytochrome c + O<sub>2</sub> + 4 H<sup>+</sup> = 4 ferricytochrome c + 2 H<sub>2</sub>O (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' ATP + L-aspartate = ADP + 4-phospho-L-aspartate (according to Swiss-Prot)  
  
* '''Protein family:''' cytochrome c oxidase subunit 3 family (according to Swiss-Prot)
+
* '''Protein family:''' aspartokinase family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P24012 P24012]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q04795 Q04795]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14910]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16760]
  
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.9.3.1 1.9.3.1]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.2.4 2.7.2.4]
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[ctaE]]''
+
* '''Operon:'''  
  
* '''[[Sigma factor]]:''' [[SigA]], [[SigE]]
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =

Revision as of 17:34, 7 June 2009

  • Description: aspartokinase I (alpha and beta subunits)

Gene name dapG
Synonyms lssD
Essential no
Product aspartokinase I (alpha and beta subunits)
Function biosynthesis of lysine and peptidoglycan
MW, pI 42 kDa, 5.709
Gene length, protein length 1212 bp, 404 aa
Immediate neighbours asd, dapA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DapG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU16760

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-aspartate = ADP + 4-phospho-L-aspartate (according to Swiss-Prot)
  • Protein family: aspartokinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed