GlpD

• Description: glycerol-3-phosphate dehydrogenase (menaquinone 7)
  
  

Gene name	glpD
Synonyms
Essential	no
Product	glycerol-3-phosphate dehydrogenase (menaquinone 7)
Function
MW, pI	62 kDa, 8.002
Gene length, protein length	1665 bp, 555 aa
Immediate neighbours	glpK, pgcA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)

• Protein family: FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): FAD

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P18158

• KEGG entry: [3]

• E.C. number: 1.1.5.3

Expression and regulation

• Operon: glpD

• Sigma factor: SigA

• Regulation: repressed by glucose (CcpA)

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

E Glatz, A Farewell, B Rutberg
The Bacillus subtilis glpD leader and antiterminator protein GlpP provide a target for glucose repression in Escherichia coli.
FEMS Microbiol Lett: 1998, 162(1);93-6
[PubMed:9595668] [WorldCat.org] [DOI] (P p)

Elisabeth Glatz, Martin Persson, Blanka Rutberg
Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA.
Microbiology (Reading): 1998, 144 ( Pt 2);449-456
[PubMed:9493382] [WorldCat.org] [DOI] (P p)

E Glatz, R P Nilsson, L Rutberg, B Rutberg
A dual role for the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability.
Mol Microbiol: 1996, 19(2);319-28
[PubMed:8825777] [WorldCat.org] [DOI] (P p)

C Holmberg, L Rutberg
An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription.
Mol Microbiol: 1992, 6(20);2931-8
[PubMed:1479885] [WorldCat.org] [DOI] (P p)

1. Glatz E, Nilsson RP, Rutberg L, Rutberg B (1996) A dual role of the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability. Mol Microbiol 19:319-328. PubMed
2. Holmberg C, Rutberg L (1992) An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription. Mol Microbiol 6:2931-2938. PubMed
3. Glatz E, Farewell A, Rutberg B: The Bacillus subtilis glpD leader and antiterminator protein GlpP provide a target for glucose repression in Escherichia coli. FEMS Microbiol Lett 1998;162:93-96. PubMed
4. Glatz E, Persson M, Rutberg B: Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA. Microbiology 1998;144:449-456. PubMed
5. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed