Difference between revisions of "YmdB"
| Line 35: | Line 35: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU16970 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 81: | Line 81: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31775 O31775] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31775 O31775] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16970] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Revision as of 12:48, 3 June 2009
- Description: unknown function
| Gene name | ymdB |
| Synonyms | |
| Essential | no |
| Product | YmdB protein |
| Function | unknown function |
| MW, pI | 29,1 kDa, 6.50 |
| Gene length, protein length | 792 bp, 264 amino acids |
| Immediate neighbours | rny, spoVS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU16970
Phenotypes of a mutant
strong overexpression of Hag
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: negative effector of the expression of hag and other members of the SigD regulon
- Protein family:
- Paralogous protein(s): similar to unknown proteins
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: O31775
- KEGG entry: [2]
- E.C. number:
Additional information
has a negative effect on hag expression
Expression and regulation
- Sigma factor:
- Regulation: constitutive
- Regulatory mechanism:
- Additional information: there is a terminator between rny and ymdB, most transcripts terminate there
Biological materials
- Mutant: GP583 (spc), available in Stülke lab
- Expression vector: pGP1040 (N-terminal His-tag, in pWH844), pGP1041 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
Jason Zemansky, Benjamin C Kline, Joshua J Woodward, Jess H Leber, Hélène Marquis, Daniel A Portnoy
Development of a mariner-based transposon and identification of Listeria monocytogenes determinants, including the peptidyl-prolyl isomerase PrsA2, that contribute to its hemolytic phenotype.
J Bacteriol: 2009, 191(12);3950-64
[PubMed:19376879]
[WorldCat.org]
[DOI]
(I p)
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
