Difference between revisions of "PolX"
| Line 35: | Line 35: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU28590 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 80: | Line 80: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94544 P94544] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94544 P94544] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU28590] |
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.7 2.7.7.7] | * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.7 2.7.7.7] | ||
Revision as of 11:57, 3 June 2009
- Description: DNA polymerase X, involved in DNA repair
| Gene name | polX |
| Synonyms | yshC |
| Essential | no |
| Product | DNA polymerase X |
| Function | DNA repair |
| MW, pI | 63 kDa, 5.308 |
| Gene length, protein length | 1710 bp, 570 aa |
| Immediate neighbours | mutSB, yshB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28590
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot) template-dependent DNA polymerase, fills single nucleotide gaps PubMed, has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates PubMed
- Protein family: DNA polymerase X family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: monomeric PubMed
- Localization:
Database entries
- Structure:
- Swiss prot entry: P94544
- KEGG entry: [2]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Margarita Salas, Madrid, Spain link
Your additional remarks
References
- Banos et al. (2008) Characterization of a Bacillus subtilis 64 kDa DNA polymerase X potentially involved in DNA repair. J. Mol. Biol. 384: 1019-1028. PubMed
- Banos et al. (2008) Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase. Nucl. Acids Res. 36: 5736-5749. PubMed
