Difference between revisions of "NadE"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU03130
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P08164 P08164]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P08164 P08164]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03130 BSU03130]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03130]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.1.5 6.3.1.5]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.3.1.5 6.3.1.5]

Revision as of 09:45, 3 June 2009

  • Description: NH3-dependent NAD+ synthetase

Gene name nadE
Synonyms outB, tscBGH, gsp-81
Essential yes PubMed
Product NH3-dependent NAD+ synthetase
Function NAD biosynthesis
MW, pI 30 kDa, 4.888
Gene length, protein length 816 bp, 272 aa
Immediate neighbours ycgI, tmrB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NadE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03130

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ (according to Swiss-Prot)
  • Protein family: NAD synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Petersohn et al. (2001) Global Analysis of the General Stress Response of Bacillus subtilis. J Bacteriol. 183: 5617-5631 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed