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* '''Description:''' nitrogen-regulated PII-like protein <br/><br/>
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#REDIRECT [[User:Aschmei]]
 
 
{| align="right" border="1" cellpadding="2"
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''nrgB''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || nitrogen-regulated PII-like protein
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of ammonium uptake
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 8.825 
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 348 bp, 116 aa
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrgA]]'', ''[[bcrC]]''
 
|-
 
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nrgB_nucleotide.txt    Gene sequence      (+200bp)  ]'''
 
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/nrgB_protein.txt Protein sequence]'''
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nrgB_context.gif]]
 
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|}
 
 
 
__TOC__
 
 
 
<br/><br/>
 
 
 
=The gene=
 
 
 
=== Basic information ===
 
 
 
* '''Coordinates:'''
 
 
 
===Phenotypes of a mutant ===
 
 
 
=== Database entries ===
 
 
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/nrgAB.html]
 
 
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10870]
 
 
 
=== Additional information===
 
 
 
 
 
=The protein=
 
 
 
=== Basic information/ Evolution ===
 
 
 
* '''Catalyzed reaction/ biological activity:'''
 
 
 
* '''Protein family:''' P(II) protein family (according to Swiss-Prot)
 
 
 
* '''Paralogous protein(s):'''
 
 
 
=== Extended information on the protein ===
 
 
 
* '''Kinetic information:'''
 
 
 
* '''Domains:'''
 
 
 
* '''Modification:'''
 
 
 
* '''Cofactor(s):'''
 
 
 
* '''Effectors of protein activity:'''
 
 
 
* '''Interactions:''' [[NrgA]]-[[NrgB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/14600241 PubMed], [[TnrA]]-[[NrgB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17001076 PubMed]
 
 
 
* '''Localization:''' cell membrane (according to Swiss-Prot),  membrane
 
 
 
=== Database entries ===
 
 
 
* '''Structure:'''
 
 
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q07428 Q07428]
 
 
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU36520 BSU36520]
 
 
 
* '''E.C. number:'''
 
 
 
=== Additional information===
 
 
 
=Expression and regulation=
 
 
 
* '''Operon:''' ''[[nrgA]]-[[nrgB]]''
 
 
 
* '''[[Sigma factor]]:''' [[SigA]]
 
 
 
* '''Regulation:''' induced by ammonium limitation
 
 
 
* '''Regulatory mechanism:''' transcription activation by [[TnrA]]
 
 
 
* '''Additional information:'''
 
 
 
=Biological materials =
 
 
 
* '''Mutant:'''
 
 
 
* '''Expression vector:'''
 
       
 
* '''lacZ fusion:'''
 
 
 
* '''GFP fusion:'''
 
 
 
* '''two-hybrid system:'''
 
 
 
* '''Antibody:''' available in [[Jörg Stülke]] lab
 
 
 
=Labs working on this gene/protein=
 
 
 
[[Susan Fisher]], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
 
 
 
=Your additional remarks=
 
 
 
=References=
 
 
 
# Atkinson MR, Fisher SH (1991) Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J Bacteriol 173:23-27. [http://www.ncbi.nlm.nih.gov/sites/entrez/1670935 PubMed]
 
# Wray, L. V., Atkinson, M. R. & Fisher, S. H. (1994). The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein. J Bacteriol 176, 108-114. [http://www.ncbi.nlm.nih.gov/sites/entrez/8282685 PubMed]
 
# Detsch, C. & Stülke, J. (2003) Ammonium utilization in ''Bacillus subtilis'': transport and regulatory functions of NrgA and NrgB. Microbiology 149: 3289-3297. [http://www.ncbi.nlm.nih.gov/sites/entrez/14600241 PubMed]
 
# Heinrich, A., Woyda, K., Brauburger, K., Meiss, G., Detsch, C., Stülke, J. & Forchhammer, K. (2006) Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in ''Bacillus subtilis''. J. Biol. Chem. 281: 34909-34917. [http://www.ncbi.nlm.nih.gov/sites/entrez/17001076 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 13:14, 25 May 2009

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