Difference between revisions of "Rnc"
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=== Basic information === | === Basic information === | ||
− | * ''' | + | * '''Locus tag:''' |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === |
Revision as of 12:59, 2 June 2009
- Description: RNase III
Gene name | rnc |
Synonyms | rncS |
Essential | yes |
Product | endoribonuclease III |
Function | cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr) |
MW, pI | 28 kDa, 8.076 |
Gene length, protein length | 747 bp, 249 aa |
Immediate neighbours | acpA, smc |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
essential PubMed
rnc is essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P51833
- KEGG entry: BSU15930
- E.C. number: 3.1.26.3
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
- Wang, W. & Bechhofer, D. H. (1997). Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci. J Bacteriol 179, 7379-7385. PubMed
- Herskovitz, M. A. & Bechhofer, D. H. (2000). Endoribonuclease RNase III is essential in Bacillus subtilis. Mol Microbiol 38:1027-1033. PubMed
- Mitra, S. & Bechhofer, D. H. (1994). Substrate specificity of an RNase III-like activity from Bacillus subtilis. J Biol Chem 269:31450-31456. PubMed
- Oguro, A., Kakeshita, H., Nakamura, K., Yamane, K., Wang, W. & Bechhofer, D. H. (1998). Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor. J Biol Chem 273:19542-19547. PubMed
- Yao, S., Blaustein, J. B. & Bechhofer, D. H. (2007). Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site. Nucleic Acids Res. 35:4464-4473. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed