Difference between revisions of "AckA"
| Line 52: | Line 52: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot) |
* '''Protein family:''' acetokinase family (according to Swiss-Prot) | * '''Protein family:''' acetokinase family (according to Swiss-Prot) | ||
Revision as of 12:18, 24 May 2009
- Description: acetate kinase
| Gene name | ackA |
| Synonyms | |
| Essential | no |
| Product | acetate kinase |
| Function | overflow metabolism |
| MW, pI | 42 kDa, 5.191 |
| Gene length, protein length | 1185 bp, 395 aa |
| Immediate neighbours | moaB, ytxK |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)
- Protein family: acetokinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P37877
- KEGG entry: BSU29470
- E.C. number: 2.7.2.1
Additional information
Expression and regulation
- Operon:
- Regulation: expression activated by glucose (2.5 fold) (CcpA) PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Your additional remarks
References
- Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
- Grundy FJ, Waters DA, Allen SH, Henkin TM (1993) Regulation of the Bacillus subtilis acetate kinase gene by CcpA. J Bacteriol 175:7348-7355 PubMed
- Turinsky, A. J., Grundy, F. J., Kim, J. H., Chambliss, G. H., and Henkin, T. M. 1998. Transcriptional activation of the Bacillus subtilis ackA gene requires sequences upstream of the promoter. J. Bacteriol. 180: 5961-5967. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
