Difference between revisions of "BglS"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[licT]]-[[bglS]]'' | + | * '''Operon:''' |
| + | ** ''[[licT]]-[[bglS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed] | ||
| + | ** ''[[bglS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed] | ||
* '''[[Sigma factor]]:''' [[SigA]] | * '''[[Sigma factor]]:''' [[SigA]] | ||
| − | * '''Regulation:''' repressed by glucose (3.1-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 | + | * '''Regulation:''' repressed by glucose (3.1-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/8245831 PubMed2], expressed in the stationary phase (temporal activation), weak induction by ß-glucosides ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed] |
| − | * '''Regulatory mechanism:''' | + | |
| + | * '''Regulatory mechanism:''' | ||
| + | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8245831 PubMed], ** [[LicT]]: binding to an RNA switch results in transcriptional antitermination [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 12:33, 16 May 2009
- Description: endo-beta-1,3-1,4 glucanase
| Gene name | bglS |
| Synonyms | bgl, licS |
| Essential | no |
| Product | endo-beta-1,3-1,4 glucanase |
| Function | lichenan degradation |
| MW, pI | 27 kDa, 6.482 |
| Gene length, protein length | 726 bp, 242 aa |
| Immediate neighbours | citH, licT |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (signal peptide) PubMed
Database entries
- Structure:
- Swiss prot entry: P04957
- KEGG entry: BSU39070
- E.C. number: 3.2.1.73 3
Additional information
Expression and regulation
- Regulation: repressed by glucose (3.1-fold) (CcpA) PubMed1 PubMed2, expressed in the stationary phase (temporal activation), weak induction by ß-glucosides (LicT) PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
- Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
- Murphy ND, McConnell DJ, Cantwell BA. 1984. The DNA sequence of the gene and genetic control sites for the excreted Bacillus subtilis enzyme β-glucanase. Nucl. Acids Res. 12:5355-5367. PubMed
- Stülke, J., Hanschke, R. & Hecker, M. (1993) Temporal activation of ß-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool. J. Gen. Microbiol. 139: 2041-2045. PubMed
- Krüger, S., Stülke, J. & Hecker, M. (1993) Carbon catabolite repression of ß-glucanase synthesis in Bacillus subtilis. J. Gen. Microbiol. 139: 2047-2054. PubMed
- Schnetz, K., Stülke, J., Gertz, S., Krüger, S., Krieg, M., Hecker, M. & Rak, B. (1996) LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family. J. Bacteriol. 178: 1971-1979. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
