Difference between revisions of "DegS"
| Line 52: | Line 52: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' autophosphorylation, autodephosphorylation, phosphorylation of [[DegU]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed] |
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 64: | Line 64: | ||
* '''Domains:''' | * '''Domains:''' | ||
| − | * '''Modification:''' phosphorylation on Ser-76 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed] | + | ** aa 12 ... 170: unknown DegS domain |
| + | ** aa 180 ... 248: HisKA_3 domain (autophosphorylation) | ||
| + | ** aa 289 ... 384: HATPase_c | ||
| + | |||
| + | * '''Modification:''' phosphorylation on Ser-76 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], autophosphorylation on His-189 | ||
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| − | * '''Effectors of protein activity:''' | + | * '''Effectors of protein activity:''' [[DegQ]] |
| − | * '''Interactions:''' [[DegS]]-[[DegU]] | + | * '''Interactions:''' [[DegS]]-[[DegU]], [[DegS]]-[[DegQ]] |
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | * '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] | ||
Revision as of 09:06, 21 May 2009
- Description: two-component sensor kinase for exoenzyme and competence regulation
| Gene name | degS |
| Synonyms | sacU |
| Essential | no |
| Product | two-component sensor kinase (NarL family) |
| Function | regulation of degradative enzyme and genetic competence |
| MW, pI | 44 kDa, 5.988 |
| Gene length, protein length | 1155 bp, 385 aa |
| Immediate neighbours | degU, yvyE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: autophosphorylation, autodephosphorylation, phosphorylation of DegU on Asp-56 PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- aa 12 ... 170: unknown DegS domain
- aa 180 ... 248: HisKA_3 domain (autophosphorylation)
- aa 289 ... 384: HATPase_c
- Modification: phosphorylation on Ser-76 PubMed, autophosphorylation on His-189
- Cofactor(s):
- Effectors of protein activity: DegQ
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- Structure:
- Swiss prot entry: P13799
- KEGG entry: BSU35500
- E.C. number:
Additional information
Expression and regulation
- Regulation: induced in the presence of ammonium (TnrA)
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Tarek Msadek, Institut Pasteur, Paris, France
Your additional remarks
References
- Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
- Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
