RpoB

• Description: RNA polymerase beta subunit
  
  

Gene name	rpoB
Synonyms
Essential	yes PubMed
Product	RNA polymerase beta subunit
Function	transcription
MW, pI	133 kDa, 4.731
Gene length, protein length	3579 bp, 1193 aa
Immediate neighbours	ybxB, rpoC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RNA polymerase beta chain family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RpoA-RpoB-RpoC

• Localization: membrane associated PubMed

Database entries

• Structure:

• Swiss prot entry: P37870

• KEGG entry: [3]

• E.C. number: 2.7.7.6

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
2. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed