Difference between revisions of "SepF"

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(Your additional remarks)
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''SepF'' mutation is synthetic lethal when combined with an ''ezrA'' mutation or an ''ftsA'' mutation.
  
 
=References=
 
=References=

Revision as of 14:39, 2 May 2009

  • Description: part of the divisome

Gene name sepF
Synonyms ylmF
Essential no
Product FtsZ-interacting protein
Function proper execution of septum synthesis
MW, pI 17 kDa, 4.863
Gene length, protein length 447 bp, 149 aa
Immediate neighbours ylmE, ylmG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YlmF context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:

Cell division

  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:

SepF binds to FtsZ

  • Localization: cytoplasm (according to Swiss-Prot)

Septum

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Leendert Hamoen, CBCB, Newcastle University, UK

Your additional remarks

SepF mutation is synthetic lethal when combined with an ezrA mutation or an ftsA mutation.

References

  1. Hamoen et al. (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division Mol. Microbiol. 59(3): 989-99. Pubmed
  2. Ishikawa et al. (2006) A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in Bacillus subtilis Mol. Microbiol. 60(6): 1364-80. Pubmed