Difference between revisions of "SacX"

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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
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|style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
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|style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: sucrose-specific phosphotransferase system,
 +
EIIBC component (low affinity)
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, control of [[SacY]] activity  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, control of [[SacY]] activity  
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__TOC__
 
__TOC__
  
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=The gene=
 
=The gene=

Revision as of 10:20, 30 April 2009

  • Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)

Gene name sacX
Synonyms ipa-14r, sacS
Essential no
Product trigger enzyme: sucrose-specific phosphotransferase system,

EIIBC component (low affinity)

Function sucrose uptake and phosphorylation, control of SacY activity
MW, pI 48 kDa, 9.346
Gene length, protein length 1377 bp, 459 aa
Immediate neighbours epr, sacY
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SacX context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induction by sucrose (at high concentration)
  • Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination)
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  2. Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed