Difference between revisions of "PGP574"

Revision as of 18:38, 7 September 2009

Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

Revision as of 12:01, 16 April 2009 (view source) CLammers (talk \| contribs) ← Older edit		Revision as of 18:38, 7 September 2009 (view source) Jstuelk (talk \| contribs) Newer edit →
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	similar [[plasmid]]: [[pGP172]]		similar [[plasmid]]: [[pGP172]]

−	Schilling, O., Herzberg, C., Hertrich, T., Vörsmann, H., Jessen, D., Hübner, S., Titgemeyer, F. & Stülke, J. (2006) Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucl. Acids Res. 34: 6102-6115. [http://www.ncbi.nlm.nih.gov/sites/~~entrez/17074746 PubMed]~~	+	<pubmed> 17074746 </pubmed>