Difference between revisions of "Sandbox"

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* '''Description:''' phospho-beta-glucosidase <br/><br/>
+
* '''Description:''' Mg2+-citrate transporter <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bglH''
+
|''citM''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yflO ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phospho-beta-glucosidase)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || Mg2+-citrate transporter
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || salicin utilization
+
|style="background:#ABCDEF;" align="center"|'''Function''' || citrate  uptake
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.957  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 6.227  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1407 bp, 469 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1299 bp, 433 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxiE]]'', ''[[bglP]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflP]]'', ''[[yflN]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/bglH_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/citM_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/bglH_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/citM_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:bglH_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:citM_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 42: Line 42:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10935]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11832]
  
 
=== Additional information===
 
=== Additional information===
Line 81: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU39260]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU07610]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 89: Line 89:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
+
* '''Operon:'''  
  
* '''Sigma factor:''' [[SigA]]
+
* '''Sigma factor:'''  
  
* '''Regulation:''' repressed by glucose ([[CcpA]]) , induced by salicin  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed], repressed by glucose (catabolite repression)
+
* '''Regulation:''' repressed by glucose ([[CcpA]])   
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression,  Induction: [[LicT]]-dependent RNA switch (antitermination), catabolite repression: repression by [[CcpA]]  ([[CcpA]] binding site overlaps -35 region) and lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]]
+
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 120:
  
 
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149  
 
# Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in ''Bacillus subtilis'': regulation of the central metabolic pathways. ''Metab Eng.'' '''5:''' 133-149  
# Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in ''Bacillus subtilis'': The ''bglP'' gene product has both transport and regulatory functions, similar to that of the ''Escherichia coli'' BglF protein. J. Bacteriol. 177: 1527-1535.  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
 
# Krüger, S. and Hecker, M. (1995) Regulation of the putative ''bglPH'' operon for aryl-ß-glycoside utilization in ''Bacillus subtilis''. J. Bacteriol. 177, 5590-5597. [http://www.ncbi.nlm.nih.gov/sites/entrez/7559347 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:42, 2 April 2009

  • Description: Mg2+-citrate transporter

Gene name citM
Synonyms yflO
Essential no
Product Mg2+-citrate transporter
Function citrate uptake
MW, pI 45 kDa, 6.227
Gene length, protein length 1299 bp, 433 aa
Immediate neighbours yflP, yflN
Gene sequence (+200bp) Protein sequence
Genetic context
CitM context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed