Difference between revisions of "AmyE"

From SubtiWiki
Jump to: navigation, search
Line 93: Line 93:
 
* '''Sigma factor:''' [[SigA]]
 
* '''Sigma factor:''' [[SigA]]
  
* '''Regulation:''' expression activated by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]  
+
* '''Regulation:''' repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  

Revision as of 22:29, 2 April 2009

  • Description: alpha-amylase

Gene name amyE
Synonyms amyA
Essential no
Product alpha-amylase)
Function starch degradation
MW, pI 72 kDa, 5.85
Gene length, protein length 1980 bp, 660 aa
Immediate neighbours ycgB, ldh
Gene sequence (+200bp) Protein sequence
Genetic context
AmyE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Henkin, T. M., Grundy, F. J., Nicholson, W. L. and Chambliss, G. H. (1991) Catabolite repression of -amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacI and galR repressors. Mol. Microbiol. 5, 575-584. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed