Difference between revisions of "TnrA"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription activator/ repressor | |style="background:#ABCDEF;" align="center"| '''Product''' || transcription activator/ repressor | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of nitrogen assimilation | + | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of nitrogen assimilation <br/>(positive regulation of nrgAB, nasBCDEF, gabP,<br/> ureABC, guaD; negative regulation of glnRA, gltAB) |
| − | |||
| − | (positive regulation of nrgAB, nasBCDEF, gabP, ureABC, guaD; | ||
| − | |||
| − | negative regulation of glnRA, gltAB) | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 10.235 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 10.235 | ||
| Line 28: | Line 24: | ||
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:tnrA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:tnrA_context.gif]] | ||
| − | |||
|- | |- | ||
|} | |} | ||
| Line 34: | Line 29: | ||
__TOC__ | __TOC__ | ||
| − | <br/><br/> | + | <br/><br/><br/> |
=The gene= | =The gene= | ||
| Line 91: | Line 86: | ||
* '''Swiss prot entry:''' | * '''Swiss prot entry:''' | ||
| − | * '''KEGG entry:''' | + | * '''KEGG entry:''' |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Revision as of 14:24, 30 March 2009
- Description: transcriptional pleiotropic regulator invoved in global nitrogen regulation
| Gene name | tnrA |
| Synonyms | scgR |
| Essential | no |
| Product | transcription activator/ repressor |
| Function | regulation of nitrogen assimilation (positive regulation of nrgAB, nasBCDEF, gabP, ureABC, guaD; negative regulation of glnRA, gltAB) |
| MW, pI | 12 kDa, 10.235 |
| Gene length, protein length | 330 bp, 110 aa |
| Immediate neighbours | mgtE, ykzB |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Genes/ operons controlled by TnrA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: feedback-inhibited GlnA prevents TnrA from DNA binding
- Interactions: TnrA-NrgB PubMed, TnrA-GlnA, this interaction results in loss of TnrA DNA-binding activity
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry:
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in the Karl Forchhammer lab
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
- Wray LV Jr, Ferson AE, Rohrer K, Fisher SH (1996) TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis. Proc Natl Acad Sci USA 93:8841-8845. PubMed
- Yoshida K, et al. (2003) Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box. Mol Microbiol 49: 157-65. PubMed
- Wray LV Jr, Zalieckas JM, Fisher SH. (2001) Bacillus subtilis glutamine synthetase controls gene expression through protein-protein interaction with transcription factor TnrA. Cell 107:427-435. PubMed
- Belitsky, B. R., and Sonenshein, A. L. (2004) Modulation of activity of Bacillus subtilis regulatory proteins GltC and TnrA by glutamate dehydrogenase. J Bacteriol 186: 3399-3407.PubMed
- Belitsky, B. R., Wray, L. V., Jr., Fisher, S. H., Bohannon, D. E. & Sonenshein, A. L. (2000). Role of TnrA in nitrogen source-dependent repression of Bacillus subtilis glutamate synthase gene expression. J Bacteriol 182, 5939-5947. PubMed
- Heinrich, A., Woyda, K., Brauburger, K., Meiss, G., Detsch, C., Stülke, J. & Forchhammer, K. (2006) Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis. J. Biol. Chem. 281: 34909-34917. PubMed
- Tojo S, Satomura T, Morisaki K, Yoshida KI, Hirooka K, Fujita Y: Negative transcriptional regulation of the ilv-leu operon for biosynthesis of branched-chain amino acids through the Bacillus subtilis global regulator TnrA. J Bacteriol 2004, 186:7971-7979. PubMed
- Fisher, S. H., Brandenburg, J. L. & Wray, L. V. (2002). Mutations in Bacillus subtilis glutamine synthetase that block its interaction with transcription factor TnrA. Mol Microbiol 45, 627-635. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
