Difference between revisions of "GltC"

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(Phenotypes of a mutant)
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=The gene=
 
=The gene=
  
== Basic information ==
+
=== Basic information ===
  
 
* '''Coordinates:'''
 
* '''Coordinates:'''
  
==Phenotypes of a mutant ==
+
===Phenotypes of a mutant ===
 
''gltC'' mutants  are auxotrophic for glutamate.
 
''gltC'' mutants  are auxotrophic for glutamate.
  
== Database entries ==
+
=== Database entries ===
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltC.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gltC.html]
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* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10810]
 
* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10810]
  
== Additional information==
+
=== Additional information===
  
 
=The protein=
 
=The protein=
  
== Basic information/ Evolution ==
+
=== Basic information/ Evolution ===
  
 
* '''Catalyzed reaction/ biological activity:''' transcription activation of the ''gltAB'' operon [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]
 
* '''Catalyzed reaction/ biological activity:''' transcription activation of the ''gltAB'' operon [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]
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* '''Paralogous protein(s):''' none, but there are 19 members of the LysR family in ''B. subtilis''
 
* '''Paralogous protein(s):''' none, but there are 19 members of the LysR family in ''B. subtilis''
  
== Extended information on the protein ==
+
=== Extended information on the protein ===
  
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
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* '''Localization:'''
 
* '''Localization:'''
  
== Database entries ==
+
=== Database entries ===
  
 
* '''Structure:'''
 
* '''Structure:'''
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18460 KEGG]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18460 KEGG]
  
== Additional information==
+
=== Additional information===
  
 
=Expression and regulation=
 
=Expression and regulation=

Revision as of 13:58, 17 December 2008

  • Description: Transcriptional activator of the gltAB operon. Activates expression of the operon in the absence of arginine.

Gene name gltC
Synonyms
Essential No
Product transcriptional regulator (LysR family)
Function positive regulation of the glutamate synthase operon (gltAB)
pI, MW 5.618, 33866.6 Da
Gene length, protein length 900 bp, 300 amino acids
Immediate neighbours gltA, proJ
Gene sequence (+200bp) Protein sequence
Genetic context
GltC context.gif











The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

gltC mutants are auxotrophic for glutamate.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation of the gltAB operon PubMed
  • Protein family: LysR-type transcription regulator PubMed
  • Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Extended information on the protein

  • Kinetic information:
  • Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation PubMed
  • Interactions:
    • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltAB operon. Note that RocG expression is strongly regulated. PubMed
  • Localization:

Database entries

  • Structure:

Additional information

Expression and regulation

  • Operon: gltC
  • Sigma factor: SigA
  • Regulation: autoregulation by GltC
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Mutant: GP344 (erm), GP738 (spc) (available in Stülke lab)
  • Expression vector: pGP903 (N-terminal His-tag) (available in Stülke lab)
  • lacZ fusion:
  • GFP fusion:
  • Antibody: available in Stülke lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

  1. Belitsky, B. R., and Sonenshein, A. L. (1995) Mutations in GltC that increase Bacillus subtilis gltA expression. J Bacteriol 177: 5696-5700.PubMed
  2. Belitsky, B. R., and Sonenshein, A. L. (2004) Modulation of activity of Bacillus subtilis regulatory proteins GltC and TnrA by glutamate dehydrogenase. J Bacteriol 186: 3399-3407.PubMed
  3. Bohannon, D. E., and Sonenshein, A. L. (1989) Positive regulation of glutamate biosynthesis in Bacillus subtilis. J Bacteriol 171: 4718-4727.PubMed
  4. Commichau, F. M., Wacker, I., Schleider, J., Blencke, H.-M., Reif, I., Tripal, P., and Stülke, J. (2007) Characterization of Bacillus subtilis mutants with carbon source-independent glutamate biosynthesis. J Mol Microbiol Biotechnol 12: 106-113. PubMed
  5. Commichau, F. M., Herzberg, C., Tripal, P., Valerius, O., and Stülke, J. (2007) A regulatory protein-protein interaction governs glutamate biosynthesis in Bacillus subtilis: The glutamate dehydrogenase RocG moonlights in controlling the transcription factor GltC. Mol Microbiol 65: 642-654. PubMed
  6. Picossi, S., Belitsky, B. R., and Sonenshein, A. L. (2007) Molecular mechanism of the regulation of Bacillus subtilis gltAB expression by GltC. J Mol Biol 365: 1298-1313. PubMed
  7. Wacker, I., Ludwig, H., Reif, I., Blencke, H. M., Detsch, C., and Stülke, J. (2003) The regulatory link between carbon and nitrogen metabolism in Bacillus subtilis: regulation of the gltAB operon by the catabolite control protein CcpA. Microbiology 149: 3001-3009.PubMed