Difference between revisions of "PWH844"
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[[File:PWH844.jpeg|250px|thumb|right|'''pWH844: click to enlarge''']] | [[File:PWH844.jpeg|250px|thumb|right|'''pWH844: click to enlarge''']] | ||
| + | [[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']] | ||
pWH844 | pWH844 | ||
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similar plasmid: [[pGP570]] | similar plasmid: [[pGP570]] | ||
| + | |||
| + | sequencing primer: | ||
| + | *'''JS39:''' 5’-TCTATCAACAGGAGTCCAAGC-3‘ | ||
| + | *'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | ||
| + | *'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | ||
| + | *'''pWH844 fw:''' 5’-TATGAGAGGATCGCATCACCAT-3‘ | ||
Schirmer et al. (1997) J. Bacteriol. '''179:''' 1329-1336. [http://www.ncbi.nlm.nih.gov/sites/entrez/9023219 PubMed] | Schirmer et al. (1997) J. Bacteriol. '''179:''' 1329-1336. [http://www.ncbi.nlm.nih.gov/sites/entrez/9023219 PubMed] | ||
Revision as of 14:56, 18 March 2009
pWH844
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
similar plasmid: pGP570
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- pWH844 fw: 5’-TATGAGAGGATCGCATCACCAT-3‘
Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed
