Difference between revisions of "PETM-11"
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* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease | * vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease | ||
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* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | * host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
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* in ''E. coli'': ampicillin resistance | * in ''E. coli'': ampicillin resistance | ||
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*from EMBL | *from EMBL | ||
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*sequencing primer: | *sequencing primer: | ||
Revision as of 09:55, 19 November 2015
- vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
- host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
- in E. coli: ampicillin resistance
- from EMBL
- sequencing primer:
- CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
- CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘
