ParA

• Description: ATPase, centromere-like function involved in forespore chromosome partitioning / negative regulation of sporulation initiation
  
  

Gene name	parA
Synonyms	soj, spo0JA
Essential	no
Product	negative regulator of sporulation initiation
Function	forespore chromosome partitioning / negative regulation of sporulation initiation
Gene expression levels in SubtiExpress: parA
Interactions involving this protein in SubtInteract: ParA
MW, pI	27 kDa, 4.903
Gene length, protein length	759 bp, 253 aa
Immediate neighbours	parB, yyaB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other, membrane proteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU40970

Database entries

• BsubCyc: BSU40970

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: regulates DNA replication initiation by either inhibiting or activating the DNA replication initiator protein DnaA PubMed; regulation of Spo0A expression PubMed

• Protein family: parA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • ParB inhibits ParA dimerization and concomitant DNA-binding activity by stimulating ParA ATPase activity PubMed

• Interactions:
  • ParA-ParB PubMed
  • ParA-DnaA PubMed

• Localization:
  • polar/ septal at the cell membrane PubMed
  • polar localization depends on the proton motive force PubMed

Database entries

• BsubCyc: BSU40970

• Structure:

• UniProt: P37522

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: parA-parB PubMed

• Expression browser: parA PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 84 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 280 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Heath Murray, Centre for Bacterial Cell Biology, Newcastle, UK homepage

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337] [WorldCat.org] [DOI] (I p)